Corning™ BioCoat™ Fibronectin Rectangular Culture Flask

Corning™ BioCoat™ products are the ideal solution for enhanced cell attachment and growth of a variety of primary cells and transformed cells in serum-free or serum-containing cultures.

Supplier:  Corning™ 354521

Catalog No. 08-774-65

Description

Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN appears to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.

• Matrix components are applied as an even, optically clear coating
• Designed to closely mimic in vivo environments and enhance expression of cell-specific morphology
• All lots tested and found negative for bacteria and fungi
• Cellware is tested for the ability to promote firm attachment of rat cerebellar granule cells

Applications include:

• Promotion of cell attachment and spreading
• Rapid expansion of cell populations
• Serum-free or reduced-serum culture
• Cell adhesion assays
• Studies of effects of HFN on cell behavior
• Improving survival of primary cells in culture

Source

• Human plasma
• Note: Source material tested for hepatitis B antigen and HIV-1 antibody

Quality Control

• Tested for ability to promote attachment and spreading of BHK-21 hamster kidney cells
• Tested and found negative for bacteria and fungi
• Fibronectin purity >90% by SDS-PAGE

Specifications

• Capacity (English): 8.45 oz.
• Capacity (Metric): 250 mL
• Neck Style: Canted
• No. per Pack: 5/Pk.
• Surface Coating: Fibronectin
• Bottom Shape: Rectangular

• Closure Material: Phenolic
• For Use With (Application): For adhesion, growth and differentiation of neuronal and transfected cell lines
• No. per Case: 10/Cs.
• Shape: Rectangular
• Sterility: Sterile
• Culture Area: 75 cm²