Invitrogen™ Human Cortisol Competitive ELISA Kit

Description

ELISA

The Human Cortisol ELISA quantitates Cortisol in human serum,plasma,urine,saliva,dried fecal extracts or or cell culture medium.

Principle of the method

The Cortisol Competitive ELISA research-use-only kit is designed to quantitatively measure cortisol present in dried fecal extracts,saliva,urine,serum,plasma,and tissue culture media samples. Total cortisol is measured in extracted samples and in serum and plasma,and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Specifications

• Assay Range: 100 to 3200 pg/mL
• Assay Sensitivity: 17.3 pg/mL
• Description: Cortisol Competitive ELISA Kit
• Assay: Multiplex assay on the Luminex Platform
• Conjugate: HRP
• Detection Method: Fluorescence
• Product Type: ELISA
• Protein Family: Steroid hormone
• Sample Type: Fecal Extract, Plasma, Saliva, Serum, Supernatant, Urine
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 8.1%
• Intraassay CV: 8.8
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Fecal Extract 50 μL, Plasma 1 μL, Saliva 12.5 μL, Serum 1 μL, Supernatant 50 μL, Urine 6.25 μL
• Storage Requirements: -20°C
• Target: Cortisol
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 30 mins