Invitrogen™ Cortisone Competitive ELISA Kit

Description

ELISA

The Cortisone ELISA quantitates Cortisone in serum,saliva,urine,plasm,or dried fecal extracts.

Principle of the method

The Cortisone Competitive ELISA research-use-only kit is designed to quantitatively measure cortisone in extracted dried fecal samples,urine,saliva,plasma,and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two-hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Cortisone (Kendall’s Compound E) is a glucocorticoid which is the product of cortisol (Compound F) deactivation by 11-beta-HSD2 (hydroxysteroid dehydrogenase), prohibiting receptor activation. The balance between cortisone and cortisol glucocorticoids helps to regulate the anti-inflammatory response. Cortisone is identical across all species.

Specifications

• Assay Range: 97.66 to 100,000 pg/mL
• Assay Sensitivity: 29 pg/mL
• Description: Cortisone Competitive ELISA Kit
• Assay: Multiplex Assay on the Luminex Platform
• Conjugate: HRP
• Detection Method: Fluorescence
• Product Type: ELISA
• Sample Type: Fecal Extract, Plasma, Saliva, Serum, Urine
• Sufficient For: 10 x 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 11.9%
• Intraassay CV: 8.6
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Fecal Extract 50 μL, Plasma 1 μL, Saliva 10 μL, Serum 1 μL, Urine 10 μL
• Storage Requirements: -20°C
• Target: Cortisone
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins