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Invitrogen™ Cortisone Competitive ELISA Kit

Catalog No. EIACORX10
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Quantity:
10 x 96 Tests
96 Tests

ELISA

The Cortisone ELISA quantitates Cortisone in serum,saliva,urine,plasm,or dried fecal extracts.

Principle of the method

The Cortisone Competitive ELISA research-use-only kit is designed to quantitatively measure cortisone in extracted dried fecal samples,urine,saliva,plasma,and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two-hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Cortisone (Kendall’s Compound E) is a glucocorticoid which is the product of cortisol (Compound F) deactivation by 11-beta-HSD2 (hydroxysteroid dehydrogenase), prohibiting receptor activation. The balance between cortisone and cortisol glucocorticoids helps to regulate the anti-inflammatory response. Cortisone is identical across all species.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 97.66 to 100,000 pg/mL
Assay Sensitivity 29 pg/mL
Description Cortisone Competitive ELISA Kit
Assay Multiplex Assay on the Luminex Platform
Conjugate HRP
Detection Method Fluorescence
Product Type ELISA
Sample Type Fecal Extract, Plasma, Saliva, Serum, Urine
Sufficient For 10 x 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 11.9%
Intraassay CV 8.6
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Fecal Extract 50 μL, Plasma 1 μL, Saliva 10 μL, Serum 1 μL, Urine 10 μL
Storage Requirements -20°C
Target Cortisone
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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