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ATCC pRS426 phagemid; in E. coli
Supplier: ATCC 77107
This is a YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. It can be used to produce ssDNA and it contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 +/- 1.4% of progeny per doubling.
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