Invitrogen™ Mouse CRG-2/CXCL10 ELISA Kit

Catalog No.	Quantity
EMCXCL10X5	5 x 96 Tests
EMCXCL10	96 Tests

Catalog No. EMCXCL10X5 Supplier Invitrogen™ Supplier No. EMCXCL10X5

Description

ELISA

The Mouse CRG-2/CXCL10 ELISA quantitates Ms CRG-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CRG-2. Principle of the method The Mouse CRG-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

Specifications

• Accession Number: P17515
• Assay Range: 15-2000 pg/mL
• Assay Sensitivity: 15 pg/mL
• Calibration Range: 15 pg/mL to 2000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL- 9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Sufficient For: 5 x 96 Assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 10 kDa interferon gamma-induced protein; 10 kDa interferon-gamma induced protein precursor; C Cmotif chemokine; C X C motif chemokine; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; chemokine C-X-C motif ligand 10; chemokine CXC-like protein; Crg2; CRG-2; CXC; CXC chemokine; CXC motif chemokine; C-X-C motif chemokine 10; C-X-C motif chemokine 10-like protein; C-X-C motif chemokine ligand 10; CXCL; CXCL10; CXCL10(1-73); gamma interferon inducible protein 10; gamma IP10; gamma-IP10; gIP-10; H-IP-10; IFI10; Inp10; interferon activated gene 10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-gamma-inducible protein 10; interferon-gamma-inducible protein-10; interferon-inducible cytokine IP-10; interferon-inducible protein 10; IP10; IP-10; M-IP-10; Mob1; mob-1; protein 10 from interferon (gamma)-induced cell line; protein Mob-1; Scyb10; similar to Small inducible cytokine B10 precursor (CXCL10) (10 kDa interferon-gamma induced protein) (Gamma-IP10) (IP-10); small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B1010 kDa interferon gamma-induced protein; 10 kDa interferon-gamma induced protein precursor; C Cmotif chemokine; C X C motif chemokine; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; chemokine C-X-C motif ligand 10; chemokine CXC-like protein; Crg2; CRG-2; CXC; CXC chemokine; CXC motif chemokine; C-X-C motif chemokine 10; C-X-C motif chemokine 10-like protein; C-X-C motif chemokine ligand 10; CXCL; CXCL10; CXCL10(1-73); gamma interferon inducible protein 10; gamma IP10; gamma-IP10; gIP-10; H-IP-10; IFI10; Inp10; interferon activated gene 10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-gamma-inducible protein 10; interferon-gamma-inducible protein-10; interferon-inducible cytokine IP-10; interferon-inducible protein 10; IP10; IP-10; M-IP-10; Mob1; mob-1; protein 10 from interferon (gamma)-induced cell line; protein Mob-1; Scyb10; similar to Small inducible cytokine B10 precursor (CXCL10) (10 kDa interferon-gamma induced protein) (Gamma-IP10) (IP-10); small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10; C7; CRG-2; INP10; IP-10; IP10; Ifi10; Scyb10; gIP-10; mob-1
• Gene ID (Entrez): 15945
• Gene Symbol: CXCL10
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: C7, CRG-2, INP10, IP-10, IP10, Ifi10, Scyb10, gIP-10, mob-1
• Target: CXCL10 (IP-10)
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min