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Sigma Aldrich Fine Chemicals Biosciences CRISPR Non-Target Negative1EA
Supplier: Sigma Aldrich Fine Chemicals Biosciences CRISPR311EA
Recent publications using CRISPR/Cas9-mediated recombineering in E. coli tout editing efficiencies near 100% making CRISPR/Cas9-mediated recombineering the most powerful bacterial genome engineering method to date. In addition Cas9-mediated recombineering overcomes the dependence on a second recombination step avoids the creation of destabilizing scar sites can be used in multiplexing and is less time-consuming than previous protocols.Here we present a novel dual-vector CRISPR/Cas-mediated»-Red system for improved recombineering in E. coli. Our system is shown to facilitate homology-directed repair of DSBs created by Cas9 endonuclease enabling genetic alterations through chromosomal integration of a donor DNA.This plasmid is to be used in combination with the Cas9 Lambda Red homologous recombination plasmid for E. coli (CAS9BAC1P) as the negative control for your custom gene editing experiment. The custom gRNA (CRISPRBACD) can be designed and ordered through https//www.sigmaald
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