Invitrogen™ Human CRP Instant ELISA™ Kit

Catalog No.	Quantity
50-182-38	128 Tests

Catalog No. 50-182-38 Supplier Invitrogen™ Supplier No. BMS288INST

Description

ELISA

The Human C-Reactive Protein (Hu CRP) ELISA quantitates Hu CRP in human serum, plasma and cell culture supernatants. The assay will exclusively recognize both natural and recombinant Hu CRP. Principle of the method The Human CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.

Specifications

• Accession Number: P02741
• Assay Range: 78-5000 pg/mL
• Assay Sensitivity: 3.0 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with Polyclonal Antibody (murine) to human C-reactive protein, HRP-Conjugate (anti human C-reactive protein monoclonal (murine) antibody) and Assay Buffer, lyophilized
  Aluminum pouch(es) with a human C-reactive protein Standard curve (coloured)
  Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Stop Solution (1M Phosphoric acid)
  Adhesive Plate Covers
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Aa1249; Ab1-341; Ab2-196; Ac1-114; Ac1262; Ac2-069; acute phase protein; CRP; AI255847; Ba2-693; c reactive; C-reactive preprotein; C-reactive protein; C-reactive protein member of the pentraxin family; C-reactive protein(1-205); C-reactive protein, member of the pentraxin family; C-reactive protein, pentraxin-related; C-reactive protein, petaxin related; CRP; CRP1; CSRP; CYRP; D1S181E; DKFZp686M148; DKFZp686M149; MGC149895; MGC88244; pentraxin 1; PTX1; RP11-419N10.4Aa1249; Ab1-341; Ab2-196; Ac1-114; Ac1262; Ac2-069; acute phase protein; CRP; AI255847; Ba2-693; c reactive; C-reactive preprotein; C-reactive protein; C-reactive protein member of the pentraxin family; C-reactive protein(1-205); C-reactive protein, member of the pentraxin family; C-reactive protein, pentraxin-related; C-reactive protein, petaxin related; CRP; CRP1; CSRP; CYRP; D1S181E; DKFZp686M148; DKFZp686M149; MGC149895; MGC88244; pentraxin 1; PTX1; RP11-419N10.4; PTX1
• Gene ID (Entrez): 1401
• Gene Symbol: Crp
• Interassay CV: 13.1%
• Intraassay CV: 6.9%
• Kit Contents: Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: -20°C
• Synonym: C reactive protein, pentraxin related, PTX1
• Target: CRP
• Test Time: 15 min
• Total Assay Time: 2 hr 10 min