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AdipoGen Crystal Violet
Supplier: AdipoGen CDXC0252G025
Chemical. CAS 548-62-9. Formula C25H30N3Cl. MW 407.98. Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M-1 cm-1. The colour of the dye depends on the acidity of the solution. At a pH of >1.0, the dye is green with absorption maxima at 420nm and 620nm, while in a strongly acidic solution the dye is yellow with an absorption maximum at 420nm. Spectral data UV/Visible Absorbance lambda max water 584-590nm. Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability, for the staining of cells to study cell migration, invasion and cell viability and as a nontoxic DNA stain for DNA visualization in agarose gels.
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