MilliporeSigma™ INS-1 832/13 Rat Insulinoma Cell Line

Description

INS-1 832/13 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.

• The rat insulinoma cell line INS-1 is a well-established model for studies of pancreatic islet beta-cell function (1); however, the GSIS response of INS-1 cells may decrease over time
• The INS-1 832/13 cell line is a subclone of INS-1 that was selected for robust GSIS, producing and secreting both rat and human insulin
• INS-1 832/13 harbors a human insulin expression cassette allowing for human insulin secretion to be maintained over extended passages with selection (2)
• The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality
• Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services
• Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination
• Each vial contains ≥ 1X10⁶ viable cells

Specifications

• Content And Storage: Store in liquid nitrogen
• Format: Vial
• For Use With (Application): INS-1 832/13 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.
• Quantity: ≥ 1 x 10⁶ cells/vial
• Source: INS-1 832/13 is a derivative of INS-1 cells originally established from an x-ray induced insulinoma in rat (1). The INS-1 832/13 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human insulin expression plasmid carrying a geneticin (G418)-resistance marker for selection (2)