Invitrogen™ Human CTACK/CCL27 ELISA Kit

Catalog No.	Quantity
EHCCL27X10	10 x 96 Tests

Catalog No. EHCCL27X10 Supplier Invitrogen™ Supplier No. EHCCL27X10

Description

ELISA

The Human CTACK/CCL27 ELISA quantitates Hu CTACK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CTACK. Principle of the method The Human CTACK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CCL27 gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation.

Specifications

• Accession Number: Q9Y4X3
• Assay Range: 10-10000 pg/mL
• Assay Sensitivity: 10 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ALP; AW558992; CC chemokine ILC; C-C motif chemokine 27; C-C motif chemokine ligand 27; Ccl27; Ccl27a; chemokine (C-C motif) ligand 27; chemokine (C-C motif) ligand 27A; CTACK; CTAK; cutaneous T-cell attracting chemokine; cutaneous T-cell-attracting chemokine; ESkine; IL-11 R-alpha-locus chemokine; IL-11 Ralpha-locus chemokine; ILC; PESKY; SCYA27; Scya27a; Skinkine; skinskine; small inducible cytokine A27; small inducible cytokine A27a; small inducible cytokine subfamily A (Cys-Cys), member 27; small-inducible cytokine A27ALP; AW558992; CC chemokine ILC; C-C motif chemokine 27; C-C motif chemokine ligand 27; Ccl27; Ccl27a; chemokine (C-C motif) ligand 27; chemokine (C-C motif) ligand 27A; CTACK; CTAK; cutaneous T-cell attracting chemokine; cutaneous T-cell-attracting chemokine; ESkine; IL-11 R-alpha-locus chemokine; IL-11 Ralpha-locus chemokine; ILC; PESKY; SCYA27; Scya27a; Skinkine; skinskine; small inducible cytokine A27; small inducible cytokine A27a; small inducible cytokine subfamily A (Cys-Cys), member 27; small-inducible cytokine A27; ALP; CTACK; CTAK; ESKINE; ILC; PESKY; SCYA27
• Gene ID (Entrez): 10850
• Gene Symbol: CCL27
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: CCL27
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min