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  7. Gibco™ ExpiCHO™ Expression Medium 

Gibco™ ExpiCHO™ Expression Medium
GREENER_CHOICE

Catalog No. p-6579008
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Quantity:
1000 mL
10 L
20 L
6 x 1 L

Description

A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells.

ExpiCHO Expression Medium is specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. This medium is GMP-manufactured, chemically-defined, serum-free, protein-free, and animal origin–free. It is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) for scalable transient protein production. To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits.

With ExpiCHO Expression Medium you can:

  • Grow suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
  • Achieve high transfection efficiencies of >90% when coupled with ExpiFectamine CHO transfection reagent
  • Transiently transfect suspension CHO cells at a density of 6 x 106 cells per mL
  • Achieve yields up to 3 g per L of recombinant protein of high-density transiently transfected cultures by sustained, high-level expression
  • Grow and transfect CHO cells at volumes of <1 mL up to 5 L in culture formats from multiwell plates up to 5 L shake flask

ExpiCHO Expression Medium is ready to use. It is formulated with GlutaMAX-I reagent and does not require supplementation. It does not contain phenol red. The chemically-defined, animal origin free formulation results in high reproducibility and lot-to-lot reliability. ExpiCHO Expression Medium is not recommended for adherent cell cultures.

TRUSTED_SUSTAINABILITY

Specifications

Cell Line ExpiCHO-S™, CHO-S, SFM Adapted
Cell Type Chinese hamster ovary (CHO) cells
Classification Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Concentration 1X
Form Liquid
Product Type CHO Expression Medium
Serum Level Serum-free
Sterility Sterile
Sterilization Method Sterile-filtered
With Additives GlutaMAX
Without Additives No Phenol Red
Product Line ExpiCHO™
Quantity 1000 mL
Shipping Condition Room Temperature
Format Bottle
Content And Storage Storage conditions: in the dark at 2-8°C
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture
Culture Type Suspension Cell Culture
Expression System ExpiCHO-S™
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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