Applied Biosystems™ Custom TaqMan™ SNP Genotyping Assays

Catalog No.	Sample Type	Quantity
A80739	Human or Non-human	2400 Reactions, Made to Order
43-320-77	Non-human	S (300 reactions), Made to Order
43-320-76	Non-human	L (2400 reactions), Made to Order
A80737	Human or Non-human	300 Reactions, Made to Order
A80738	Human or Non-human	1000 Reactions, Made to Order

Catalog No. A80739 Supplier Applied Biosystems™ Supplier No. A80739

Description

Provide a highly flexible technology for detection of polymorphisms within any genome.

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5'-nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). Assays contain one probe labeled with VIC or Cyanine 5 dye to detect the Allele 1 sequence; the second probe is labeled with FAM or ABY dye to detect the Allele 2 sequence.

Custom TaqMan SNP Genotyping Assays can be easily designed at no extra charge by submitting target sequences confidentially to our secure Custom Assay Design Tool.

Benefits:

• Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
• Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
• Flexible—obtain assay designs for any SNP in the human genome using our secure Custom Assay Design Tool, at no extra charge
• Tested—all custom human assays (VIC/FAM SKUs only) are subjected to a functional test on 20 unique DNA samples. Assays ordered with non-human or Cyanine 5/ABY SKUs are not functionally tested.
• Multiplexing ready—TaqMan assays are now available with with VIC/FAM or Cyanine 5/ABY formats to support simultaneous detection of two sets of SNPs (two pairs of alleles).

Approximate ship time

4–6 days in North America and 6–10 days in Europe (VIC/FAM); 4-10 days in North America, 6-14 days in Europe (Cyanine 5/ABY)

The free Custom Assay Design Tool can generate assays for the detection of SNPs, MNPs, and indels of up to six bases. These custom assays are designed, synthesized, formulated, optimized, and quality control tested.

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (singleplex) or TaqPath ProAmp Master Mix (multiplex) (sold separately).

All assay designs are the product of our industry-leading bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355) or TaqPath ProAmp Master Mix (Cat. No. A30865)

Specifications

• Quantity: 2400 Reactions, Made to Order
• Shipping Condition: Room Temperature
• For Use With (Application): Genotyping
• Sample Type: Human or Non-human
• Key Functions: Cyanine 5 - MGB probe detects Allele 1, ABY - MGB probe detects Allele 2
• For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne, StepOnePlus, ViiA 7 System, QuantStudio Absolute Q Digital PCR System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals.
• Product Line: TaqMan
• Content And Storage: •1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers).
  •Store at -15°C to -25°C.
• No. of Reactions: 2400 Reactions, Made to Order
• Format: Tube
• Concentration: 80X
• Product Type: SNP Genotyping

Frequently Asked Questions (FAQs)

How do I determine if Applied Biosystems TaqMan Assays that I want to multiplex are compatible with each other?

You can utilize the Applied Biosystems TaqMan Multiplex Interaction Check (MIC) tool. The MIC tool uses bioinformatics to identify potential interactions between primers, probes, and amplicons that could negatively impact multiplex qPCR performance. The tool was developed and validated using data from approximately 200 multiplex panels, combining computational analysis with wet-lab testing. By flagging assay combinations that may be prone to false positives or reduced sensitivity, MIC tool helps researchers design multiplex panels with greater confidence and reduce time spent on trial-and-error optimization.risk and observed multiplex performance.

How many Applied Biosystems TaqMan SNP Genotyping Assays can I multiplex in a single reaction?

You can multiplex up to two Applied Biosystems TaqMan SNP Genotyping Assays to interrogate two sets of alleles. The first assay will utilize FAM/VIC dyes, while the second assay will utilize ABY/Cyanine 5 dyes.

For Research Use Only. Not for use in diagnostic procedures.