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Crystal Violet Erythromycin Agar (CVE) is an enriched, selective, and differential medium used for the isolation and presumptive identification of Fusobacterium nucleatum. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.CVE has been formulated with lower concentrations of pancreatic digest of casein and glucose than most media, to provide less optimal conditions for fastidious microorganisms. Tryptophan, yeast extract, and defibrinated sheep blood are added to increase the recovery of F. nucleatum from clinical specimens. This medium contains erythromycin and crystal violet at concentrations that inhibit most other anaerobes, but has no suppressive effects on F. nucleatum. When grown on CVE, F. nucleatum colonies grow to approximately 2.0 mm and has a blue pigmentation.