Invitrogen™ Human CXCL16 ELISA Kit

Catalog No.	Quantity
EHCXCL16X5	5 x 96 Tests
EHCXCL16	96 Tests

Catalog No. EHCXCL16X5 Supplier Invitrogen™ Supplier No. EHCXCL16X5

Description

ELISA

The Human CXCL16 ELISA quantitates Hu CXCL16 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CXCL16. Principle of the method The Human CXCL16 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses. The chemokine domain of CXCL16 contains six cysteine residues, including the four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids. The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal mucin-like stalk sequence. The extracellular domain contains 89 amino acid residues (86 a.a. residues for the murine homolog).

Specifications

• Accession Number: Q9H2A7
• Assay Range: 3-2000 pg/mL
• Assay Sensitivity: 3 pg/mL
• Calibration Range: 3 pg/mL to 2000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 5 x 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 0910001K24Rik; AV290116; b2b498Clo; BB024863; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 16; CXC; Cxc chemokine ligand 16; CXC motif chemokine; C-X-C motif chemokine 16; C-X-C motif chemokine ligand 16; CXCL; Cxcl16; CXCL16v1; CXCL16v2; CXCLG16; Mutant line 498; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; Srpsox; SR-PSOX; SR-PSOX/CXCL16; Transmembrane chemokine CXCL16; UNQ2759/PRO6714; zinc finger, MYND-type containing 15; Zmynd150910001K24Rik; AV290116; b2b498Clo; BB024863; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 16; CXC; Cxc chemokine ligand 16; CXC motif chemokine; C-X-C motif chemokine 16; C-X-C motif chemokine ligand 16; CXCL; Cxcl16; CXCL16v1; CXCL16v2; CXCLG16; Mutant line 498; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; Srpsox; SR-PSOX; SR-PSOX/CXCL16; Transmembrane chemokine CXCL16; UNQ2759/PRO6714; zinc finger, MYND-type containing 15; Zmynd15; CXCLG16; SR-PSOX; SRPSOX
• Gene ID (Entrez): 58191
• Gene Symbol: Cxcl16
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Inflammation
• Sample Volume: Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: UNQ2759/PRO6714, CXCLG16, SR-PSOX, SRPSOX
• Target: CXCL16
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min