Specific detection system for simultaneous analysis of hypoxia and oxidative stress using microscopy and flow cytometry. Enzo Life Sciences’ ROS-ID.The Hypoxia (Red) dye takes advantage of the nitroreductase activity present in hypoxic cells by converting the Nitro group to hydroxylamine (NHOH) and amino (NH.The Oxidative Stress Detection Reagent is a non-fluorescent, cell-permeable total ROS detection dye which reacts directly with a wide range of reactive species. The generated fluorescent products can be visualized using a wide-field fluorescence microscope equipped with standard fluorescein (490/525 nm) and Texas Red (596/670 nm) filters, confocal microscopy, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection, HTS. Contents: Hypoxia Red Detection Reagent. Oxidative Stress Detection Reagent (Green). ROS Inducer (Pyocyanin). Hypoxia Inducer (DFO)