The Human Cytochrome P450 2E1 (CYP2E1) ELISA Kit is designed to quantitatively measure CYP2E1 levels in serum plasma tissue homogenates and cell lysates using a Sandwich ELISA method This kit detects CYP2E1 with a sensitivity of 10 94 pg/mL and offers a broad detection range from 43 75 pg/mL to 2800 pg/mL ensuring accurate measurement across diverse sample concentrations The assay requires a sample volume of 50-100 L and delivers results within 1-5 hours depending on protocol optimization Absorbance is measured at 450 nm to determine CYP2E1 concentration providing a reliable and standardized approach for research applications Its robust design accommodates various biological matrices making it suitable for studies focused on drug metabolism toxicology or CYP2E1-related disease mechanisms