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Cell Biolabs Inc CytoSelect™ Leukocyte Transmigration Assay Kit (Fluorometric), 24 assays
Supplier: Cell Biolabs Inc CBA212
Leukocyte extravasation into perivascular tissue requires leukocyte interaction with vascular endothelium and consists of multiple, consecutive processes including the capture of leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration. This multistep process is realized by sequential activation-dependent interactions between endothelial cell adhesion molecules and their ligands on leukocytes. The first step of transient adhesion is mediated by an interaction of leukocyte or endothelial cell selectins and their oligosaccharide-bearing ligands. Arrest and firm adhesion of leukocytes to endothelium depends on β2 integrin activation on the leukocyte cell surface, followed by interaction with endothelial cell proteins. The CytoSelect™ Leukocyte Transmigration Assay provides a robust system for the quantitation of leukocyte-endothelium interactions and transmigrations. The kit contains sufficient reagents for the evaluation of 24 assays in a 24-well plate.
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