Invitrogen™ Human D-Dimer ELISA Kit

Catalog No.	Quantity
EHDDIMERX5	5 x 96 Tests
EHDDIMER	96 Tests
EHDDIMERX10	10 x 96 Tests

Catalog No. EHDDIMERX5 Supplier Invitrogen™ Supplier No. EHDDIMERX5

Description

ELISA

The Human D-Dimer ELISA quantitates Hu D-Dimer in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu D-Dimer. Principle of the method The Human D-Dimer solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream.

Specifications

• Accession Number: P02671
• Assay Range: 0.082-60 pg/mL
• Assay Sensitivity: 0.08 pg/mL
• Calibration Range: 0.08 pg/mL to 60 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cell Structure/Organelle Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human D-Dimer. Other species not determined.
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: carboxy-terminal region; ENSMUSG00000059807; FGA; Fib; Fib2; fibrinogen A-alpha-chain; fibrinogen alpha chain; fibrinogen, A alpha polypeptide; Fibrinopeptide A; MGC119422; MGC119423; MGC119425carboxy-terminal region; ENSMUSG00000059807; FGA; Fib; Fib2; fibrinogen A-alpha-chain; fibrinogen alpha chain; fibrinogen, A alpha polypeptide; Fibrinopeptide A; MGC119422; MGC119423; MGC119425; Fib2
• Gene ID (Entrez): 2243
• Gene Symbol: FGA
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: Fib2
• Target: D-dimer
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min