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AdipoGen DAN
Supplier: AdipoGen CDXD0062G001
Chemical. CAS 771-97-1. Formula C10H10N2. MW 158.2. Synthetic. The 2,3-diaminonaphthalene DAN assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity Ex/Em ~365/415nm. However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline DAN is used as a derivatisation-reagent for the determination of selenium at low detection limits.
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