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Abcam DAN, Fluorescence determination of NO2-, 5G

Supplier:  Abcam AB1452485G

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MW 158.2 g/mol, Purity >98%. Routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay uses the reaction of DAN with NO₂- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity. Also used as a derivatisation-reagent for the determination of selenium at low detection limits.

The product is subject to the following: Abcam Restricted Use Statement

Catalog No. 50-270-5361


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