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AdipoGen Dansyl chloride (5 g)
Supplier: AdipoGen CDXD0043G005
Chemical. CAS: 605-65-2. Formula: C12H12ClNO2S. MW: 269.75. Synthetic. Dansyl chloride is a fluorogenic probe that reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green-fluorescent sulfonamide adducts. It is used for protein sequencing and N-terminal amino acid and peptide analysis by reverse phase high performance liquid chromatography (HPLC). It can also be used to label hydroxyl and carboxylic acid functional groups. Dansyl chloride is non-fluorescent until it reacts with amines. The resulting Dansyl amides have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property, in combination with their ability to accept energy (as in fluorescence resonance energy transfer) from the amino acid tryptophan, has made Dansyl chloride an important tool for biophysical studies.
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