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Sigma Aldrich Fine Chemicals Biosciences DAPI, 28718-90-3, MFCD00012681, 1 mg
Supplier: Sigma Aldrich Fine Chemicals Biosciences D95421MG
Empirical Formula (Hill Notation): C16H15N5 · 2HCl, Molecular Weight: 350.25, Synonym: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, 4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI dihydrochloride, Assay ≥98% (HPLC and TLC), for nucleic acid staining. DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
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