BD Biosciences™ DAPI Solution (RUO)

Description

Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.

• Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.
• DAPI is predominantly impermeant to live cells, allowing it to be used as a viability dye in unfixed cells to discriminate intact from membrane-compromised cells.
• Note, however, that high concentrations of the dye may still enter intact cells.
• Additionally, DAPI may be used to analyze DNA content in fixed cells, or as a nuclear counterstain in imaging or flow cytometry.
• When bound to double-stranded DNA, DAPI has an excitation wavelength maximum of 358 nm and an emission maximum of 461 nm.
• DAPI is also well excited by the violet laser line (eg, 405 nm). Note that DAPI also binds RNA.
• Under these conditions, DAPI emits maximally at 500 nm, but with less intensity than when bound to double-stranded DNA.

Specifications

• Concentration: 1.0 mg/mL
• Stain Type: Nucleic acid stain
• For Use With (Application): Flow cytometry, bioimaging, immunofluorescence
• Quantity: 1 mg
• Storage Requirements: Avoid multiple freeze-thaws of product. The product should be kept undiluted at -20°C for long term storage, and it may be kept undiluted at 4°C for short term storage.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov