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Dojindo Molecular Technologies Inc DAPRed – Autophagy Detection (5 nmol)
Supplier: Dojindo Molecular Technologies Inc D67710
DAPRed is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. The small fluorescent molecule DAPRed is used to detect autophagosomes and autolysosomes. The mechanism suggests that the dye is incorporated into the autophagosome during double-membrane formation via structural features and then emits fluorescence under hydrophobic conditions. DALRed is cell-permeable, has no requirement of transfection method, and enables live-cell imaging with fluorescence microscopy. Excitation and emission of DAPRed: λex: 530 nm, λem: 720 nm. For monitoring only autolysosomes, we recommend DALGreen [D675] as it enables the detection of phagosome-lysosome fusion. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b)
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