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Vector Laboratories DSL, Fluorescein, 2 mg L-1030-5
Supplier: Vector Laboratories L10305
Datura Stramonium Lectin (DSL), Fluorescein, 2 mg, is a conjugated form of the DSL lectin. This lectin contains two chains of 40 kDa and 46 kDa joined by disulfide bonds and is free of a reported 32 kDa contaminant protein. The carbohydrate binding site recognizes (beta-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (beta-1,6) and (beta-1,2) was reported to be the most potent inhibitor of agglutination. Fluorescein-labeled DSL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin and is supplied essentially free of unconjugated fluorochromes. Fluorescein has an excitation maximum of 495 nm and an emission maximum of 515 nm.
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