Sigma Aldrich Fine Chemicals Biosciences dCas9 3XfLAGTM Biotin Prot

Supplier:  Sigma Aldrich Fine Chemicals Biosciences DCAS9PROT250UG

Recombinant dCas9-3X fLAG-Biotin protein from Streptococcus pyogenes (~165 KD) is a ready-to-use reagent for genome engineering and genomic DNA detection isolation and imaging experiments. When combined with target-specific guide RNAs dead Streptococcus pyogenes Cas9 protein acts as a DNA targeting protein suitable for transfection of cell cultures microinjection DNA pull-down chromatin enrichment microbial identification and in situ DNA imaging. At the C terminus the protein is tagged with a SV40 large T antigen nuclear localization signal (NLS) for nuclear import and both a 3XfLAG epitope and biotin for anti-fLAG(R)-based or streptavidin-based experiments.

Catalog No. 50-290-5013