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Enzo Life Sciences 2,7-Dichlorodihydrofluorescein diacetate (50mg). CAS: 4091-99-0
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Supplier:  Enzo Life Sciences ALX610022M050

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Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=59,500M-1cm-1). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF. Alternative name: DCDHF diacetate, 2',7'-Dichlorofluorescein diacetate. Purity: ≥95% (HPLC). Solubility: 25mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers. Long Term Storage: -20°C.

Catalog No. 50-200-9594


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