Abnova™ DDIT3 Split CISH Probe

Catalog No.	For Use With (Application)
89-335-538	CISH-P

Catalog No. 89-335-538 Supplier Abnova™ Supplier No. CS0005

Description

DDIT3 Split CISH Probe is designed for the qualitative detection of human DDIT3 gene at 12q13.3 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (distal to the DDIT3 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the DDIT3 breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the DDIT3 gene region, two red/green fusion signals appear.
Aberrant situation: One DDIT3 gene region affected by a translocation is indicated by one separate green signal and one separate red signal.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P