The DEAE-Dextran Transfection Kit contains the cationic polymer transfection reagent DEAE-Dextran that can be applied for cell transfection DEAE-Dextran can complex negatively-charged DNA to form complexes which are internalized into cells by endocytosis to achieve gene expression This transfection reagent is suitable for plasmid DNA transfection of various adherent or suspension cell types The transfection kit has advantages such as ease of operation and cost-effectiveness Additionally the kit provides Chloroquine which inhibits lysosomal DNA degradation thereby increasing transfection efficiency This reagent is mainly used for transient gene expression rather than for the establishment of stable cell lines Moreover under high concentrations or prolonged incubation periods this reagent may cause cytotoxicity Therefore condition optimization is required to achieve optimal results