Gibco Defined Keratinocyte-SFM (1X)
Defined Keratinocyte-SFM (1X)
Manufacturer: Gibco 10744019
DescriptionDefined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM) allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.
This formulation contains some human and animal origin components.
A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.
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|Keratinocytes, epithelial cells|
|For the isolation and expansion of human keratinocytes, cell culture, mammalian cell culture|
|Defined Keratinocyte-SFM Medium|
|2 to 8°C|
|2 to 8°C|
|Defined Keratinocyte-SFM Medium, 500mL, Liquid Form, 2 to 8°C Storage Temperature, 10 Weeks Shelf Life, Serum-Free, For Isolation and Expansion of Human Keratinocytes|
|L-glutamine, phenol red|
Safety and Handling
ShelfLife : 10 Week(s)
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For Research Use Only. Not for use in diagnostic procedures.