Gibco™ Defined Keratinocyte SFM (1X)   

Defined Keratinocyte-SFM (1X)

Manufacturer:  Gibco™ 10744019

Catalog No. 10-744-019

Description

Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM) allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.

Note:
This formulation contains some human and animal origin components.

Note:
A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.

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Specifications

• Serum Level: Serum-Free
• Form: Liquid
• Phenol Red Indicator: Phenol Red
• Content And Storage: • 500 mL Defined Keratinocyte-SFM Basal Medium; store in refrigerator (2–8°C) and protect from light
  • 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5 to -20°C) and protect from light
  
  Complete Medium: store in refrigerator (2–8°C) and protect from light.

• Species: Human
• Glutamine: L-Glutamine
• Classification: Serum-Free
• Cell Type: Keratinocytes, Epithelial Cells

For Research Use Only. Not for use in diagnostic procedures.