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Gibco™ Defined Keratinocyte-SFM (1X) Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Defined Keratinocyte-SFM (1X)

Manufacturer:  Gibco™ 10744019

Catalog No. 10-744-019


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Description

Description

Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM) allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.

Note:
This formulation contains some human and animal origin components.

Note:
A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.

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Specifications

Specifications

Keratinocytes, epithelial cells
For the isolation and expansion of human keratinocytes, cell culture, mammalian cell culture
Liquid
Defined Keratinocyte-SFM Medium
2 to 8°C
2 to 8°C
Defined Keratinocyte-SFM Medium, 500mL, Liquid Form, 2 to 8°C Storage Temperature, 10 Weeks Shelf Life, Serum-Free, For Isolation and Expansion of Human Keratinocytes
Liquid
1 Each
500mL
L-glutamine, phenol red
Safety and Handling

Safety and Handling

ShelfLife : 10 Week(s)

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For Research Use Only. Not for use in diagnostic procedures.