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Gibco™ Defined Keratinocyte SFM (1X)


Description
Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM) allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.
Note:
This formulation contains some human and animal origin components.
Note:
A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.

Specifications
Specifications
Cell Type | Keratinocyte Cells, Epithelial Cells |
Classification | Serum-free |
Concentration | 1 X |
Form | Liquid |
Product Type | Kit |
Serum Level | Serum-free |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | Glutamine, Phenol Red |
Quantity | 500 mL |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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