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Sigma Aldrich Fine Chemicals Biosciences Denaturation Solution for
Supplier: Sigma Aldrich Fine Chemicals Biosciences N15314L
Sigmas denaturing SOLUTIONon is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination SOLUTIONon treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination allowing for more efficient transfer of large DNA in neutral Southern transfers.
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