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Applied Biosystems™ dGTP BigDye™ Terminator v1.0 Ready Reaction Cycle Sequencing Kit

Catalog No. 4307175
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100 Reactions
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43-071-75 100 Reactions
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Catalog No. 43-071-75 Supplier Applied Biosystems™ Supplier No. 4307175
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Description

Includes

Contains AmpliTaq™ DNA Polymerase, FS, BigDye terminators v1.0, pGEM™ - 3Zf(+) Control Template, -21 M13 Control Primer (forward), and Premixed Ready Reaction.

dGTP BigDye™ terminator cycle sequencing kit with the AmpliTaq™ DNA polymerase, FS. This ready reaction kit uses the same dye set as the BigDye™ v1.0 kits.

  • Ensure reliable results with difficult regions and finishing applications
  • Sequence templates with challenging sequence contexts
  • Sequence problematic templates that standard chemistries cannot
  • Optimized for GT-Rich templates: Obtain higher success rate even with your difficult GT-rich templates
  • By using the same chemistries as the standard BigDye™ terminator kits (only replacing dITP with dGTP) the dGTP BigDye™ terminator kit provides proven performance
  • Optimized for GT-rich and other difficult-to-sequence templates
  • Easily Integrate into your workflow: dGTP BigDye™ terminator kits require no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols
  • The dGTP BigDye™ terminator kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples

Order Info

100 rxns

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage The kit contains the AmpliTaq™ DNA Polymerase, FS, BigDye
terminators v1.0, pGEM™ - 3Zf(+) Control Template,
-21 M13 Control Primer (forward), and Premixed Ready Reaction.
Store kit at -15 to -25°C.
Format 12-well Strips, 8-well Strip, 96- or 384-well Plate
For Use With (Application) GT-Rich or Difficult Template Sequencing
No. of Reactions 100 reactions
Product Line BigDye Terminator, BigDye
Product Type Sequencing Kit
Quantity 100 Reactions
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Genomic DNA (Bacterial), Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA

For Research Use Only. Not for use in diagnostic procedures.

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