Sigma Aldrich Fine Chemicals Biosciences DIG High Prime DNA Labelin

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 11745832910

The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG) asteroid hapten to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. This kit contains a ready-made blocking SOLUTIONon combined stock SOLUTIONon of of nitroblue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme nucleotides primers and reaction buffer all in one convenient reagent. The sample material can be DNA fragments of at least 100bp linearized plasmid cosmid or lambdaDNA or supercoiled DNA. The quotrandom primedquot DNA labeling method originally developed by feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA and is not degraded during the reaction making it possible to label minimal amountsof DNA (10n

Catalog No. 50-290-5247