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Enzo Life Sciences Digoxigenin-dUTP, alkali-stable (25nmol)
Supplier: Enzo Life Sciences ENZNUC1130025
Digoxigenin-dUTP replaces dTTP in reactions in which it serves as a substrate for E. coli DNA polymerase , T4 and Taq DNA polymerases, reverse transcriptase and terminal transferase. Digoxigenin-dUTP can be used to produce digoxigenin-labeled DNA probes in a variety of labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The resulting digoxigenin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots. The probes can also be used for in situ hybridization procedures on fixed cells and tissues. Digoxigenin-labeled probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-labeled probes. Alternative name: Digoxigenin-11-dUTP. Concentration: 1mM. Quantity: Sufficient for approximately 25 reactions, following the recommended protocol of ENZ-GEN111. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water.
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