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AdipoGen Dihydrorhodamine 123
Supplier: AdipoGen CDXD0134M125
Chemical. CAS 109244-58-8. Formula C21H18N2O3. MW 346.38. Synthetic. Cell-permeable non-fluorescent reactive oxygen species ROS indicator. Passively diffuses across membranes where it is oxidized by peroxynitrite to rhodamine 123 which localizes in the mitochondria and exhibits green fluorescence Ex/Em wavelengths of 500 and 536 nm. Used to investigate reactive oxygen intermediates produced by human and murine phagocytes, activated rat mast cells and cultured endothelial cells. In addition, it has been used together with Fura Red calcium indicator to simultaneously measure oxidative bursts and Ca2 fluxes in monocytes and granulocytes. Dihydrorhodamine 123 has been shown to be a more sensitive probe than H2DCFDA for detecting granulocyte respiratory bursts.
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