Invitrogen™ Dihydrotestosterone Competitive ELISA Kit

Catalog No.	Quantity
EEL221	96 Tests

Catalog No. EEL221 Supplier Invitrogen™ Supplier No. EEL221

Description

ELISA

Dihydrotestosterone Competitive ELISA Kit quantifies Dihydrotestosterone in serum and plasma. The Dihydrotestosterone Competitive ELISA research-use-only kit is designed to quantitatively measure DHT. A DHT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to DHT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of DHT in the samples is then determined by comparing the OD of the samples to the standard curve.. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Dihydrotestosterone (DHT) is a biologically active metabolite of the hormone testosterone, formed primarily in the prostate gland, testes, hair follicles, and adrenal glands by the enzyme 5-alpha-reductase by means of reducing the alpha 4, 5 double-bond. Dihydrotestosterone belongs to the class of compounds called androgens, also commonly called androgenic hormones or testoids. DHT is thought to be approximately 30 times more potent than testosterone because of increased affinity to the androgen receptor. [National Center for Biotechnology Information. PubChem Compound Database; CID=10635].

Specifications

• Assay Range: 39.06-2500 pg/mL
• Assay Sensitivity: 23.44 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Chemical
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Andractim; Androlone; Androstanolone; C19H30O2; DHT; STANOLONEAndractim; Androlone; Androstanolone; C19H30O2; DHT; STANOLONE;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis, Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Shipping Condition: Wet Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Dihydrotestosterone
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min