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Biotium DiO/DPA membrane potential detection kit
Supplier: Biotium 30037
The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a "stationary" FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change. The DiO/DPA membrane potential detection kit includes a vial of the green fluorescent membrane dye DiOC16(3) in DMSO (1 mL at 2 mM) and a vial of the anionic membrane quencher dye DPA (dipicrylamine) in DMSO (1 mL at 20 mM). DiO has an excitation/emission of 484/501 nm and DPA has an excitation of 406 nm.
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