Thermo Scientific Chemicals DL-1,4-Dithiothreitol, 99%, for biochemistry

Description

1,4-Dithiothreitol, CAS # 3483-12-3, is a reducing agent commonly used to reduce disulphide bonds in protein studies, such as western blot SDS-page electrophoresis. | CAS: 3483-12-03 | C₄H₁₀O₂S₂ | 154.25 g/mol

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General description

• 1,4-Dithiothreitol (DTT or Cleland's reagent) reduces disulfide bonds in proteins, leading to loss of native conformation
• DTT is not stable in solution and should be prepared in small aliquots prior to use; the use of EDTA in the solution can increase the half-life of DTT
• Typically used at a final concentration of 0.1M for protein stabilization; recommended to create a 1M stock

Applications

• Protects thiol groups from oxidation and acts as a chemical chelator in solution; maintains proteins in the denatured state
• Common applications include SDS-page electrophoresis and protein isolation

Specifications

Chemical Identifiers

• CAS: 3483-12-03
• MDL Number: MFCD00004877
• InChI Key: VHJLVAABSRFDPM-IMJSIDKUSA-N
• Synonym: dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt
• PubChem CID: 446094
• ChEBI: CHEBI:42170
• IUPAC Name: (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
• SMILES: C(C(C(CS)O)O)S

• Absorbance: (1 M soln. in water), (1 M soln. in water) at 0nm, 0.035 max. at 500nm
• Melting Point: 39°C to 44°C
• Color: White
• Infrared Spectrum: Authentic
• Assay Percent Range: 0.99
• Molecular Formula: C4H10O2S2
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• Quantity: 1 g
• Beilstein: 01, III, 2360
• Merck Index: 15, 3419
• Transmittance: 99% min. (c=5, 660nm, water) 1cm cell
• Molecular Weight (g/mol): 154.24
• Formula Weight: 154.24
• Percent Purity: ≥98.5%
• Grade: Biochemical
• Physical Form: Powder
• Chemical Name or Material: DL-1, 4-Dithiothreitol

Safety and Handling

Hazard Category	Serious eye damage/eye irritation (category 2), Acute toxicity (category 4), Specific target organ toxicity after single exposure (category 3), Skin corrosion/irritation (category 2)
Hazard Statement	Causes serious eye irritation, Harmful if swallowed, May cause respiratory irritation, May form combustible dust concentrations in air, Causes skin irritation

SDS

RUO – Research Use Only