Dl-Dithiothreitol, MP Biomedicals™

Description

Used to stabilize sulfhydryl-containing enzymes. Dl-Dithiothreitol, MP Biomedicals reduces protein disulfide bonds in sample buffers prior to SDS-PAGE. DTT is also used to reduce the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to brea

DTT is a Clelands reagent that acts as a protective agent for sulfhydryl groups (-SH). It quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide, which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

Specifications

Chemical Identifiers

• CAS: 27565-41-9
• Molecular Formula: C4H10O2S2
• Molecular Weight (g/mol): 154.24
• MDL Number: MFCD00004877,MFCD00064305
• InChI Key: VHJLVAABSRFDPM-UHFFFAOYNA-N
• Synonym: 1,4-Dithiothreitol, DTT, 1,4-Dithio-DL-threitol, Cleland Reagent racemic, (±)-threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent
• IUPAC Name: 1,4-disulfanylbutane-2,3-diol
• SMILES: OC(CS)C(O)CS

• Melting Point: 40°C to 50°C
• pH: 4.0 to 6.0 (0.1M in H₂O, 25°C) (literature)
• Quantity: 250 mg
• Solubility Information: Soluble in water (50mg/mL, clear and colorless solution), ethanol, acetone, ethylate, chloroform and ether.
• Formula Weight: 154.2
• Grade: Molecular Biology Reagent
• Physical Form: Powder

Safety and Handling

Recommended Storage

Store at 4°C.