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Dl-Dithiothreitol, MP Biomedicals™

Catalog No. MP21948212 Shop All MP Biomedicals, Inc Products
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MP21948212 250 mg
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Catalog No. MP21948212 Supplier MP Biomedicals, Inc Supplier No. 02194821.2
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Description

Used to stabilize sulfhydryl-containing enzymes. Dl-Dithiothreitol, MP Biomedicals reduces protein disulfide bonds in sample buffers prior to SDS-PAGE. DTT is also used to reduce the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to brea

DTT is a Clelands reagent that acts as a protective agent for sulfhydryl groups (-SH). It quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide, which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 27565-41-9
Molecular Formula C4H10O2S2
Molecular Weight (g/mol) 154.24
MDL Number MFCD00004877,MFCD00064305
InChI Key VHJLVAABSRFDPM-UHFFFAOYNA-N
Synonym 1,4-Dithiothreitol, DTT, 1,4-Dithio-DL-threitol, Cleland Reagent racemic, (±)-threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent
IUPAC Name 1,4-disulfanylbutane-2,3-diol
SMILES OC(CS)C(O)CS

Specifications

Melting Point 40°C to 50°C
pH 4.0 to 6.0 (0.1M in H2O, 25°C) (literature)
Quantity 250 mg
Solubility Information Soluble in water (50mg/mL, clear and colorless solution), ethanol, acetone, ethylate, chloroform and ether.
Formula Weight 154.2
Grade Molecular Biology Reagent
Physical Form Powder

Safety and Handling

Recommended Storage Store at 4°C.
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