DL-Dithiothreitol, MP Biomedicals™

Description

DL-Dithiothreitol, Electrophoresis grade, ≥99.5%, White Powder

• Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes.
• Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
• DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
• DTT is less pungent and is less toxic than 2-mercaptoethanol.
• DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH).
• In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution.

Specifications

Chemical Identifiers

• CAS: 3483-12-3
• Molecular Formula: C4H10O2S2
• Molecular Weight (g/mol): 154.24
• MDL Number: MFCD00004877
• InChI Key: VHJLVAABSRFDPM-IMJSIDKUSA-N
• Synonym: 1,4-Dithiothreitol, DTT, 1,4-Dithio-DL-threitol, Cleland Reagent

• Melting Point: 40°C to 50°C
• Color: White
• pH: 4.0 to 6.0 (0.1 M in H2O, 25°C)
• Flash Point: 113°C / 235.4°F (lit.)
• Quantity: 5 g
• UN Number: UN3335
• Beilstein: 1719757
• Solubility Information: Soluble in water (50mg/mL - clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether.
• Grade: ≥99.5%
• Physical Form: Powder

Safety and Handling

RTECSNumber	EK1610000
storageNote1	+4°C