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DL-Dithiothreitol, MP Biomedicals™

Catalog No. MP21005972
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Quantity:
250 mg

Description

Known as Clelands reagent. DL-Dithiothreitol, MP Biomedicals is a protective agent that quantitatively reduces disulfides (-S-S- to -SH). In this reaction, DTT is oxidized to the cyclic disulfide, ensuring the reduction of other disulfides in solution. Di

Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DTT has been used:

  • As one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk
  • As a component of medium for the demembranation and reactivation of spermatozoa
  • To maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst
  • As a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 3483-12-3
Molecular Formula C4H10O2S2
Molecular Weight (g/mol) 154.24
InChI Key VHJLVAABSRFDPM-IMJSIDKUSA-N
Synonym dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt
PubChem CID 446094
ChEBI CHEBI:42170
IUPAC Name (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
SMILES C(C(C(CS)O)O)S

Specifications

Melting Point 40°C to 50°C (literature)
pH 4.0 to 6.0
Quantity 250 mg
Solubility Information Soluble in water (50mg/mL, clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether.
Formula Weight 154.2
Percent Purity ≥99.0%
Physical Form Powder

Safety and Handling

Recommended Storage Store at 4°C
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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