TOPOGEN INC DNA Gyrase Drug Screening Kit

Supplier:  TOPOGEN INC TG2001G1KIT

This drug screening Kit is based on gyrase supercoiling action on a relaxed plasmid DNA substrate provided (pHOT1, which is a derivative of pBR322) and roughly 2.7 KB. One unit of gyrase will supercoil 200-500 ng in 1 hr at 37oC. The enzyme introduces negative supercoils into a relaxed pHOT1 plasmid substrate via a sign inversion model. An energy co-factor (ATP) is required for the complete supercoiling reaction. Non-EB gels are ideal for detecting gyrase activity as attested by the excellent resolution between supercoiled and relaxed DNA forms. It is designed to identify IFP and CIC (poisons and inhibitors, respectively). A typical IFP (e.g., a fluoroquinolone) will induce double strand DNA breaks while a prototypical CIC will simply block the enyzme from acting on relaxed DNA (novobiocin, some intercalators for example). Thus, an IFP (ciprofloxacin) will result in formation of a linear DNA product while a CIC will block supercoiling activity.

Catalog No. NC2216107