Invitrogen™ Human DNA-PK Native Protein

Description

Native Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Native human protein purified from MOLT-4 cells. No special measures were taken to activate this kinase. Protein Form: Full Length

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

Specifications

• Accession Number: P78527
• Concentration: Conc. Not Determined
• For Use With (Application): Kinase Assay
• Formulation: 25 mM HEPES with 0.2 mM EDTA, 10% glycerol, 10 mM MgCl2, 1 mM DTT, 50 mM KCl and no preservative; pH 7.5
• Gene ID (Entrez): 5591
• Molecular Weight (g/mol): 600 kDa
• Name: Human DNA-Pk.
• Quantity: 10 μg
• Gene Alias: AI326420; AU019811; DNA-dependent protein kinase catalytic subunit; DNAPDcs; DNAPK; DNA-Pk. catalytic subunit; DNA-PKcs; DNPK1; DOXNPH; doxorubicin nephropathy; dxnph; hyper-radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; I79_017613; IMD26; p350; p460; Prkdc; protein kinase, DNA activated, catalytic polypeptide; protein kinase, DNA-activated, catalytic polypeptide; scid; severe combined immunodeficiency; slip; Xrcc7
• Common Name: DNA-Pk.
• KinaseFamily: PIKK Kinase Family
• Conjugate: Unconjugated
• Species: Human
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical terbium phospho-specific activity
• Kinase Group: Ser⁄Thr Kinases: Atypical
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™
• Target Entry: DNAPK
• Target Gene: DNA-Pk.
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length
• Form: Liquid
• Protein Subtype: Lipid Kinases
• Research Category: Signal Transduction