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Genscript Corporation DNase I

Catalog No. 5000058948
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Deoxyribonuclease I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA double-stranded DNA and DNA-RNA hybrids yielding 5 -phosphate-terminated polynucleotides with a free hydroxyl group on position 3 producing tetranucleotides The activity of DNase I is strictly dependent on Ca2 and can be activated by divalent metal ions such as Mg2 or Mn2 In the presence of Mg2 DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite on either strand and in the presence of Mn2 it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with 1 2 nucleotide overhangs GenScript is offering DNase I produced by expression in a P pastoris strain carrying a plasmid encoding the bovine DNase I

Catalog No. 50-000-58948 Supplier Genscript Corporation Supplier No. E000535000
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Deoxyribonuclease I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA double-stranded DNA and DNA-RNA hybrids yielding 5 -phosphate-terminated polynucleotides with a free hydroxyl group on position 3 producing tetranucleotides The activity of DNase I is strictly dependent on Ca2 and can be activated by divalent metal ions such as Mg2 or Mn2 In the presence of Mg2 DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite on either strand and in the presence of Mn2 it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with 1 2 nucleotide overhangs GenScript is offering DNase I produced by expression in a P pastoris strain carrying a plasmid encoding the bovine DNase I

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