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Sigma Aldrich Fine Chemicals Biosciences DNase I Amplification Grad

Catalog No. 502900012
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Deoxyribonuclease I (DNase I) is an endonucleaseisolatedfrom bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides. Amplification Grade DNase I has been purified to remove RNase activity and is suitable for eliminating DNA from RNA preparations prior to sensitive applications such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction). DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop SOLUTIONon. Heating also denatures hairpins in the RNA so the RNA can be used directly in reverse transcription.No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laborator

Catalog No. 50-290-0012 Supplier Sigma Aldrich Fine Chemicals Biosciences Supplier No. AMPD11KT
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Deoxyribonuclease I (DNase I) is an endonucleaseisolatedfrom bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides. Amplification Grade DNase I has been purified to remove RNase activity and is suitable for eliminating DNA from RNA preparations prior to sensitive applications such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction). DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop SOLUTIONon. Heating also denatures hairpins in the RNA so the RNA can be used directly in reverse transcription.No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laborator

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