Invitrogen™ Donkey anti-Chicken IgY (H+L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluor™ 568

Catalog No.	Quantity
A78950	500 μg

Catalog No. A78950 Supplier Invitrogen™ Supplier No. A78950

Description

Donkey Polyclonal Secondary Antibody

This donkey anti-chicken IgY (H+L) whole secondary antibody has been affinity-purified and shows minimum cross-reactivity to bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondary antibodies flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g. flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 568 dye is a bright, orange/red-fluorescent dye with excitation ideally suited to the 568 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 568 dye molecules can be attached...

Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Chicken IgY (H+L) Highly Cross Adsorbed
• Applications: Peptide Array, Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 2 mg/mL
• Conjugate: Alexa Fluor 568
• Formulation: PBS with 5mM sodium azide; pH 7.5
• Host Species: Donkey
• Immunogen: Purified Chicken IgY, Heavy and Light chains.
• Purification Method: Purified
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Chicken
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG