Invitrogen™ Donkey anti-Rabbit IgG (H+L) Secondary Antibody, AP

Donkey Polyclonal Secondary Antibody

Supplier:  Invitrogen™ A16026

Catalog No. A16026

Description

The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG (H+L)
• Classification: Polyclonal
• Conjugate: Alkaline Phosphatase (AP)
• Host Species: Donkey
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Rabbit
• Product Type: Secondary Antibody
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry
• Concentration: 2.5 mg/mL
• Formulation: secondary antibody with 1% BSA, 5mM MgCl2, 30mM triethanolamine, 0.1mM ZnCl2 and 0.05% sodium azide; pH 7.2
• Immunogen: Gamma Immunoglobins Heavy and Light chains.
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid