Invitrogen™ DPPIV (DPP4/CD26) Mouse ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EMDPP4

Catalog No. EMDPP4

Description

The Mouse DPPIV (DPP4/CD26) ELISA quantitates Ms DPPIV in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms DPPIV. Principle of the method The Mouse DPPIV solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti CD3-induced IL - 2 production. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.

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Specifications

• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Product Type: ELISA Kit
• Assay: Solid-phase sandwich ELISA
• Substrate Type: TMB
• Label or Dye: HRP
• Assay Range: 0.41-100 ng/mL
• Description: DPPIV (DPP4) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Proteases, Peptidases and Inhibitors Protein Family, Cell Cycle and Proliferation Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Dppiv (Dpp4) i
• Gene ID (Entrez): 13482
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr. 45 min.
• Detectable Analytes: DPPIV (DPP4)
• Research Discipline: Cell Cycle & Proliferation
• Interassay CV: <12%
• Quantity: 96 Tests
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: Cd26,Dpp-4,THAM
• Detection Method: Colorimetric

• For Use With (Equipment): Colorimetric Microplate Reader
• Sufficient For: 96 Assays
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• Accession Number: P28843
• Calibration Range: 0.45ng/mL to 100ng/mL
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Mouse DPPIV precoated 96-well strip plate, lyophilized recombinant mouse DPPIV standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent A, contains 0.
• Sample Volume: Plasma, 5 μL; Serum, 5 μL; Supernatant, 100 μL
• Test Time: 1 hr. 20 min.
• Target: Mouse
• Host Species: Mouse
• Synonym: RP23-87G8.2, Cd26, Dpp-4, THAM
• Intraassay CV: <10%
• Target Species: Mouse
• Assay Sensitivity: 0.45 ng/mL
• Immunoassay Kit Format: Solid