AdipoGen DTNB

Supplier:  AdipoGen CDXD0156G005

Chemical. CAS 69-78-3. Formula C14H8N2O8S2. MW 396.35. Synthetic A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins from any solvent accessible Cys under mild alkaline conditions pH 7-8 produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm Extinction coefficient 14150 M-1*cm-1.

Catalog No. 50-256-1209