Biotium Dual Apoptosis Assay with NucView 488 caspase-3 Substrate and Texas Red-Annexin V (50 - 250 assays)

Supplier:  Biotium 30030

These kits provides two apoptosis markers, our novel NucView 488 Caspase-3 Substrate and Annexin V conjugated with a dye, for profiling apoptotic cells and studying the relation of two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation. Annexin V conjugation for the second marker is available with the following dyes: Sulforhodamine 101 (Texas Red) Annexin V, CF594 Annexin V, and CF640R Annexin V. Unlike conventional caspase assays, NucView 488 Caspase-3 substrate detects caspase-3 activity within individual whole cells in a non-interfering manner. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. The kit contains reagents sufficient for 50 flow cytometry assays (200 uL assay volume).

Catalog No. 50-196-4484